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Author: Estec Marketing


As ESTEC Ltd runs its FTIR promotion, here a few things to note about the equipment.

FTIR Explained
First, Fourier Transform Infrared Spectroscopy is a technique used to obtain an infrared spectrum of absorption or emission of a solid, liquid, or gas and in the process identify known and unknown materials/samples by utilizing Infrared Electromagnetic Radiation. An FTIR spectrometer simultaneously collects high-resolution spectral data over a wide spectral range giving it an advantage over conventional spectrophotometers.

In principle, the molecules vibrate, bonds stretch and bend when they absorb IR Radiation. This is done by passing a beam of IR light through a sample. Some of this light will be reflected while others will be absorbed by the sample and consequently specific light will travel through the sample carrying molecular information of the sample. The transmitted light then goes through a detector where the information is interpreted and transformed to a spectrum (sample spectrum). This is achieved by the Fourier Transform mathematical process hence the acronym FTIR. Identification occurs when the sample spectrum is compared to a reference/known spectrum. I know you want to ask how it happens and/or where the known spectrum is. Well wonder not…

All our FTIRs come with LabSolutions IR – An analytical Data system for FTIR. LabSolutions IR is furnished with a rich Spectral Library (Approx. 12,000 spectra of organic compounds, polymers, pharmaceutical products, inorganic compounds, food additives, contaminants, etc.) and a High-performance search function as standard.

We also offer, with our FTIR’s, Attenuated Total Reflectance (ATR) Accessory – Single-Reflection Accessory with a Diamond Crystal. The accessory enables the measurement of a wide variety of samples regardless of their form or properties. I.e., Solids, Liquids, and gels in the mid and far-infrared. Measurement is made without destroying the sample as no sample preparation is required.

As conveyed in the posters, the choice of a particular Model depends on your application, and If I may add, your future needs.


FTIR’s Comparison
Look at the table below showing the Differentiation between our FTIR series based on customer needs.

Item IRTracer-100 Affinity-1S IRSpirit
S/N Ratio 60,000:1 30,000:1 30,000:1
Wavenumber Expansion Suitable for customers with absorption of the desired component in the near or far infrared regions Suitable for customers who analyze only in the mid-infrared region Suitable for customers who analyze only in the mid-infrared region
Contaminant Analysis Suitable for customers who want to connect infrared microscope to analyze minute foreign matter or plan to add infrared microscope in the future Suitable for customers who want to connect infrared microscope to analyze minute foreign matter or plan to add infrared microscope in the future Suitable for customers who do not plan to connect infrared microscope to analyze foreign matter up to the size that can be handled.
High Sensitivity Suitable for customers who want to analyze with a highly sensitive MCT detector – used when analyzing minute or low transmittance samples. Suitable for customers who do not require an MCT detector Suitable for customers who do not require an MCT detector
Go for if you are… Research, Academic, Forensic, Environment Research, Academic, Forensic, Environment Pharma


These are just but a few points, talk to us for more details, we are happy to advise on the model that will best suit your application.

And In addition to the above, please note that we have two more new models; IRXross and FTIR Plastic Analysis System.

The Plastic Analyzer method package includes an FTIR spectral library for plastics degraded by UV rays and heat. Utilizing searches of this library demonstrates its effectiveness in the analysis of unknown samples that are difficult to identify with standard libraries. Examples include plastics degraded by exposure to UV rays as well as contaminants and defective items altered by heating.

The IRXross is a mid-level FTIR developed by downgrading IRTracer-100. Performance is almost the same as IRTracer-100 except for wavenumber expandability and S/N ratio which is 55,000:1.

Till next time…..

Written by: Serah Katini- Sales Executive, ESTEC Ltd

Published by: Mitchelle GacheriMarketing Executive, ESTEC Ltd


Liquid chromatography (LC) separates the components of a sample based on the differences in their affinity/retention strength during the stationary phase and mobile phase. Common LC techniques, namely reversed-phase, normal phase, and size exclusion chromatography. With technological advancements the LC has evolved to analyze smaller particle sizes and higher pressure that are more efficient, have higher speed, sensitivity, and resolution. This includes the high-performance liquid chromatography (HPLC) and the ultrahigh-performance liquid chromatography (UHPLC).

Upon separation by LC, the components can be detected using optical properties such as ultraviolet-visible (UV-VIS), fluorescence, refractive index, evaporative light scattering, or electrical conductivity based on the analytes’ properties. When the analyte passes through the detector, a change (e.g., increase or decrease) in the optical property will be observed and recorded.

Chromatograms obtained using these optical detectors primarily identify or qualify substances based on the retention time and quantitate substances based on the peak area and intensity. The LC chromatogram shows an example of a typical chromatogram obtained using these optical detectors. LC coupled with optical detection offers great quantitative accuracy for analytes that can be chromatographically resolved, where a detected peak comprises only a single component. However, achieving required resolution is challenging for complex samples where multiple components elute approximately at the same time. In contrast, mass spectrometry (MS) offers a highly sensitive detection technique that ionizes the sample components, separates the resulting ions in vacuum based on their mass-to-charge ratios (m/z), and measures the intensity of each ion. A mass spectrum plots the relative ion intensities against the m/z values, and a series of mass spectra are generated at each time point. This information indicates the concentration level of ions that have a given mass and is extremely valuable for the unique identification of molecules, also known as qualitative analysis. Moreover, MS provides added specificity and sensitivity, and the convenience of simultaneous multicomponent analysis.


When the LC is coupled with MS, the mass spectra obtained from these measurements provide molecular mass and structural information for eluted components, which supplement the qualitative information based on retention times obtained using other LC detectors. Therefore, LCMS combine the outstanding separation resolution of LC with the excellent qualitative capabilities of MS.

With its superior qualitative and quantitative capabilities and robustness, LCMS is commonly used to meet the rigorous demands of the analytical market and industries. LCMS is applied in many industries such as pharmaceuticals, biopharmaceuticals, forensic, industrial, food and environmental sector. For clinical research, the analysis of drugs, vitamins and minerals in whole blood, plasma, serum and urine is conducted routinely using LCMS. It is also applied in metabolomics, proteomics and genomics study. The use of LCMS in the biopharmaceutical discipline have enabled the bioanalysis and characterization of antibody drugs.


In the environmental field, LCMS is widely utilized for the qualitative and quantitative determination of known pollutants (e.g. pesticides, bacteria, pharmaceuticals and personal care products) and trace-level emerging contaminants. Food safety and development have also adopted the use of LCMS in their product quality control such as the quantitation of residual veterinary drugs, food additives and the composition analysis of supplements and organic foods. With high sensitivity, high detection selectivity and high qualitative capability, MS bring about the flexibility of simultaneous multi-component analysis, improved productivity, and efficiency to HPLC analyses in these applications.


Written By Steven Kuria – Sales Executive ESTEC Ltd 
Published By Mitchelle Gacheri – Marketing Executive ESTEC Ltd